Publications/CRISPR related


27. Zhao, X., Li, S., Liu, G., Wang, Z., Yang, Z., Zhang, Q., Liang, M., Liu, J., Li, Z., Tong, Y., Zhu, G., Wang, X., Jiang, L., Wang, W.*, Tan, G.Y.*, Zhang, L.* A versatile biosensing platform coupling CRISPR–Cas12a and aptamers for detection of diverse analytes. Science Bulletin. doi: 10.1016/j.scib.2020.09.004 (2020). Read more…


25. Blin, K., Shaw, S., Tong, Y., Weber, T.* Designing sgRNAs for CRISPR-BEST base editing applications with CRISPy-web 2.0. Synth. Syst. Biotechnol. 5, 99-102. doi: 10.1016/j.synbio.2020.05.005 (2020). Read more…


24. Tong, Y., Whitford, C. M., Blin, K., Jørgensen, T. S., Weber, T.*, Lee, S. Y.* CRISPR-Cas9-based, CRISPRi, and CRISPR-BEST-mediated genetic manipulation in streptomycetes. Nat. Protoc. 15, 2470–2502. doi: 10.1038/s41596-020-0339-z (2020). Read more…


22. Wang, Q., Xie, F., Tong, Y., Habisch, R., Yang, B., Zhang, L.*, Müller, R.*, Fu, C.* Dual-function chromogenic screening-based CRISPR/Cas9 genome editing system for actinomycetes. Appl. Microbiol. Biotechnol. 104, 225-239. doi: 10.1007/s00253-019-10223-4 (2020). Read more…


21. Tong, Y., Whitford, C. M., Robertsen, H. L., Blin, K., Jørgensen, T. S., Klitgaard, A. K., Weber, T.*, Lee, S. Y.* Highly efficient DSB-free base editing for streptomycetes with CRISPR-BEST. Proc. Natl. Acad. Sci. U.S.A., 116, 20366-20375. doi: 10.1073/pnas.1913493116 (2019). Read more…


20. Liang, M., Li, Z., Wang, W., Liu, J., Liu, L., Zhu, G., Karthik, L., Wang, M., Wang, K-F., Wang, Z., Yu, J., Shuai, Y., Yu, J., Zhang, L., Yang, Z., Li, C., Zhang, Q., Shi, T., Zhou, L., Xie, F., Dai, H., Liu, X., Zhang, J., Liu, G., Zhuo, Y., Zhang, B., Liu, C., Li, S., Xia, X., Tong, Y., Liu, Y., Alterovitz, G., Tan, G-Y.*, Zhang, L.* A CRISPR-Cas12a-derived biosensing platform for the highly sensitive detection of diverse small molecules. Nat. Commun. 10, 3672. doi: 10.1038/s41467-019-11648-1 (2019).  Read more…


18. Tong, Y., Weber, T.*, Lee, S. Y.* CRISPR/Cas-based genome engineering in natural product discovery. Nat. Prod. Rep. 36, 1262-1280. doi: 10.1039/C8NP00089A (2019). Journal cover paper. Read more…


17. Tong, Y., Robertsen, H. L., Blin, K., Weber, T.*, Lee, S. Y.* CRISPR-Cas9 Toolkit for Actinomycete Genome Editing. Methods Mol. Biol. 1671, 163-184. doi: 10.1007/978-1-4939-7295-1_11 (2018). Read more…


14. Weber, T.*, Tong, Y., Blin, K., Lee, S. Y. Engineering of actinomycetes using CRISPR/Cas9 technologies. Planta Medica. 81, S01. doi: 10.1055/s-0036-1596147 (2016). Read more...


13. Tong, Y., Charusanti, P., Zhang, L., Weber, T.*, Lee, S. Y.* CRISPR-Cas9 Based Engineering of Actinomycetal Genomes. ACS Synth. Biol. 4, 1020-1029. doi: 10.1021/acssynbio.5b00038 (2015). Journal cover paper. ESI highly cited paper. Read more…