Protocols

Here you can find a step-by-step video clip on E. coli-streptomyces conjugation to deliver CRISPR system into your target streptomyces strain [1].

Reference:

  1. Tong, Y., Whitford, C. M., Blin, K., Jørgensen, T. S., Weber, T.*, Lee, S. Y.* CRISPR-Cas9-based, CRISPRi, and CRISPR-BEST-mediated genetic manipulation in streptomycetes. Nat. Protoc. 15, 2470–2502. doi: 10.1038/s41596-020-0339-z (2020).